Meanwhile, the application of magnetic microsphere in biomedical field such as enzyme immobilization, cell separation, targeted medicine and diagnosis were narrated in detail. 同时着重阐述了磁性微球在固定化酶、向药物、胞分离、断等生物医学领域的应用。
A Disposable Organophosphorus Pesticides Enzyme Biosensor Based on Composite Nano-particles and Magnetic Separation with Enrichment Modified Screen-printed Carbon Electrodes 基于复合纳米微粒修饰和磁性分离富集的一次性有机磷农药酶传感器
Methods Cells were isolated from pseudomembrane around failing prosthesis by enzyme digestion and then separated into CD14+ and CD14-cells by immunomagnetic bead separation. 方法采用酶消化法从假膜组织中分离细胞,应用免疫磁珠法将细胞分离成CD14+和CD14-细胞并在各种条件下分别培养。
The cytoplasm and intercellular fluid can be extracted from enzyme hydrolyzed cellulosic material and pectin, then the laminarin can be obtained via separation and purification. 应用酶解技术将纤维质和果胶质水解,提取出细胞质和细胞间液,再经分离纯化可提取海带多糖。
The soybean as main material was used to manufacture soybean milk by dry method ( choice, peeling, enzyme deactivation, simple grinding, fine grinding, and separation). 以优质大豆为主要原料,经干法生产(大豆筛选、脱皮、高温灭酶、粗磨、超细研磨、浆渣分离)制得豆奶;
Chitosanase was produced by fermentation of two steps method of mycelium culture and induced enzyme generation separation. 使用菌丝培养和诱导产酶两步分离法发酵生产壳聚糖酶。通过产酶条件研究发现氨基葡萄糖是产酶的有效诱导物。
Enzyme engineering technology can improve the extraction and separation efficiency as well as transformation of active components in traditional Chinese medicine ( TCM). 利用酶工程技术,可以提高中药有效成分的提取率,促进微量活性成分的转化。
Methods To isolate human mesenteric artery smooth muscle cells with acute enzyme separation method and record the electric current of the calcium-activated potassium channels in these cells using patch clamp single channel technique. 方法用急性酶分离法分离人肠系膜平滑肌细胞,采用单通道膜片钳技术记录该细胞上的钙激活钾通道电流。
Application of Aqueous Two-Phase Technology in Enzyme Separation 双水相技术在酶分离纯化中的运用
Several kinds of preparation methods and the applied developments of magnetic polymer microsphere in cell separation, immobilized enzyme, DNA separation and medicine carrier are discussed in detail, and the prospects of magnetic polymer microsphere are also proposed. 在此基础上,对磁性高分子微球在细胞分离、固定化酶、靶向药物、核酸分离等领域的最新应用及存在的问题进行了分析,并指出了该领域今后的研究方向。
The application of some advanced food technologies, including microwave assisted extraction technology, resin adsorption technology, enzyme technology, membrane separation technology and vacuum freeze drying technology in pectin preparation were discussed in this paper. 简述了微波萃取技术、树脂吸附技术、酶技术、膜分离技术、真空冷冻干燥技术等若干食品加工新技术在果胶制备中的应用。
It benefits the reuse of enzyme and separation the production and enzyme The simply developing history character and immobilized method is reviewed. 本文综述了固定化酶的发展简史、性质和固定方法,介绍了固定化酶中应用的载体。
In recent years, considerable research attention has been focused on the environment-sensitive hydrogels ( intelligent hydrogel) because they possess great potential applications in many aspects, including drug delivery system, enzyme immobilization and biomaterials separation and purification, etc. 壳聚糖的改性及其智能水凝胶的研究环境敏感性水凝胶(智能水凝胶)在药物控制释放、酶的固定化及生物物质分离提纯等方面有着诱人的应用前景,因而近年来受到普遍关注。
The kind of reactor is product facility or experimental installation which makes use of semipermeable membrane for separating product from enzyme, and it will combine the process of separation, purification and recycle. Furthermore, the reactor is capable of running continuously. 该类反应器是利用半透膜分离生物酶和反应产物的生产设备或实验装置,是反应与分离耦合的装置,集产品分离、纯化和酶回收再利用于一体,可实现连续化操作。
This article has offered sach advance biological technology and separation means as biological enzyme, flocculant, membrane separation and resin combinative foramen adsorption in order to extract the total saponins of Panax notoginseng. 介绍生物酶工程、絮凝技术、膜分离、大网格组合大孔吸附树脂技术在水提取三七总甙中的应用,提出中药水提取、分离工艺的新思路和新方法。
The Hydrolysis of Microbial Oil Catalyzed by Enzyme and Concentration Separation of the Ethyl Esters of Poly-unsaturated Fatty Acids 生物油脂的酶催化水解及其脂肪酸乙酯的浓缩分离
Applications of Enzyme Technology in Separation and Purification of Functional Lipids 酶反应技术在功能性油脂分离和纯化中的应用
Effects of feed velocity, operational pressure and enzyme activity of xylanases on ultrafiltration separation of endo-β-xylanase and β-xylosidase were investigated. 研究了进料速度、操作压力、木聚糖酶活大小对内切-β-木聚糖酶和β-木糖苷酶超滤分离的影响。
Objective: To summarize the application of the following seven technologies to the analysis of internal Chinese drugs in the modernization of traditional Chinese medicine ( TCM): supercritical fluid extraction, capillary electrophoresis, macroporous adsorption resin, biochip, TCM fingerprint, enzyme and membrane separation. 目的:综述中药现代化7种高新技术:超临界流体萃取技术、毛细管电泳技术、大孔吸附树脂技术、生物芯片技术、中药指纹图谱技术、酶技术、膜分离技术在体内药物分析中的应用。
The interaction ways between Tb3+ and horse radish peroxidase ( HRP) were investigated for the first time using UV-Vis absorption spectroscopy, fluorescence spectroscopy, atomic absorption spectroscopy and enzyme separation technique. 利用紫外可见吸收光谱、红外光谱、荧光光谱、原子吸收及酶分离方法,首次研究了稀土离子Tb3+与植物辣根过氧化物酶(HRP)的相互作用方式。
The functional magnetic particles are widely used in enzyme immobilization, cells and protein separation, drug targeting, wastewater treatment, etc., due to their good magnetic responsiveness and surface functionality. 功能化磁性粒子同时兼具磁响应性和表面功能性,被广泛地应用于酶的固载、细胞和蛋白质分离、靶向给药、污水处理等领域。
In order to solve the problem, attempts on purification process, protein concentration assay and enzyme activity assay were practiced, which would contribute to the separation of low concentration proteins. 针对这一问题,在纯化操作,蛋白含量测定和酶活性测定方法上都进行了有效的尝试和改进,这为今后分离低含量的蛋白酶积累了经验。
Among that, magnetic materials as a common functional materials is more in a wide range of applications in biomedical and biotechnology, such as enzyme immobilization, protein and immune determination, the purification of RNA and DNA, cell separation and magnetic targeted drugs [ 6-9]. 而磁性材料作为一种常见的功能性材料在生物医学和生物技术方面更有着广泛的应用,如蛋白质和酶的固定化、免疫测定、RNA和DNA的提纯、细胞分离及磁性靶向药物。
Compared with free enzymes, the stability of the immobilized enzyme increases, can use repeatedly, easy and reactant apart, be helpful for the separation and purification of products, so as to improve the quality of products. 与自由酶相比,固定化酶的稳定性增加、可以反复使用、易与反应物分开,有利于产物的分离纯化,从而提高产品质量。
Methods: The culture of tissue block and enzyme digestion was used for the SF separation respectively. Besides, every passage of SF cells was carefully observed under microscope, and the curves of their growth were measured as well. 方法:分别采用组织块法、酶消化法分离培养SF,对不同代次SF细胞进行相差显微镜观察、生长曲线测定。
Because of the water-soluble and unstable properties of the free state enzyme, it usually needs to be immobilized on the carrier to improve the diffusion of the zymolyte molecule, enhance the thermodynamic stability of enzyme and facilitate the separation and reusage of the enzyme. 游离态酶一般溶于水、不稳定,将酶固定在载体上,有利于底物分子的扩散,提高酶的热力学稳定性,并方便分离和重复使用。
Conclusion: Enzyme digestion method is proved to be a better way for cell separation and culture in tissue engineering study, and the first to the fourth passage of SF cells can be used as seeding cells in the study of ACL tissue engineering. 结论:酶消化法分离培养SF细胞更适合于组织工程,SF细胞第一~第四代可作为构建组织工程化前交叉韧带的种子细胞。
However, Low content of the enzyme in the body, so separation and purification is difficult. 但是,该酶在体内含量低,分离纯化困难。